ggt [2018-11-01 12:40:21]

gamma-glutamyltransferase

locus: BSU18410

pI: 5.45

mw: 64.01 kDa

protein length: 587 aa

gene length: 1761 bp

function: degradation of poly-glutamate capsules

product: gamma-glutamyltransferase

essential: no

ec: 2.3.2.2

synonyms: pac

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.4|Capsule biosynthesis and degradation]
• [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 2,004,677 → 2,006,440

The protein

Catalyzed reaction/ biological activity

• (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid (according to Swiss-Prot)

Structure

• [PDB|2V36]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|5C31D968E3786858B025CE02CFAC48F61700031F|ggt]
  description: [Pubmed|22383849]

  regulation

  • strongly induced in response to glucose starvation in M9 medium [Pubmed|23033921]

  additional information

  • [protein|search|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• BKE18410 (Δ[gene|5C31D968E3786858B025CE02CFAC48F61700031F|ggt]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTCCCTCCTATATG, downstream forward: _UP4_TAAATAAAAAACTGTACTCG
• BKK18410 (Δ[gene|5C31D968E3786858B025CE02CFAC48F61700031F|ggt]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTCCCTCCTATATG, downstream forward: _UP4_TAAATAAAAAACTGTACTCG

References

• 27095459,15583164,12892879,18957862,20088880,22383849,23335395,23033921,21513304,21987357,24279353,24531494,24719567,29685354